{rfName}

Indexed in

License and use

Citations

42

Altmetrics

Analysis of institutional authors

Conti SCorresponding AuthorTrepat XCorresponding AuthorLabernadie AAuthor
Share
Publications
>
Article

CAFs and cancer cells co-migration in 3D spheroid invasion assay

Publicated to:Crispr Knock-Ins In Organoids To Track Tumor Cell Subpopulations. 2179 243-256 - 2021-01-01 2179(), DOI: 10.1007/978-1-0716-0779-4_19

Authors: Conti, S; Kato, T; Park, D; Sahai, E; Trepat, X; Labernadie, A

Affiliations

Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Barcelona, Spain - Author
CIBER Bioingenieria, Biomateriales y Nanomedicina - Author
Ctr Networked Biomed Res Bioengn Biomat & Nanomed, Barcelona, Spain - Author
Francis Crick Inst, London, England - Author
Inst Catalana Rec & Estudis Avancats ICREA, Barcelona, Spain - Author
Institució Catalana de Recerca i Estudis Avançats - Author
Institute for Bioengineering of Catalonia - Author
Instituto de Bioingeniería de Cataluña - Author
Integrative Cell and Tissue Dynamics. Institute for Bioengineering of Catalonia - Author
The Francis Crick Institute - Author
Univ Barcelona, Fac Med, Unitat Biofis & Bioengn, Barcelona, Spain - Author
Universitat de Barcelona - Author
See more

Abstract

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. In many solid tumors, collective cell invasion prevails over single-cell dissemination strategies. Collective modes of invasion often display specific front/rear cellular organization, where invasive leader cells arise from cancer cell populations or the tumor stroma. Collective invasion involves coordinated cellular movements which require tight mechanical crosstalk through specific combinations of cell–cell interactions and cell–matrix adhesions. Cancer Associated Fibroblasts (CAFs) have been recently reported to drive the dissemination of epithelial cancer cells through ECM remodeling and direct intercellular contact. However, the cooperation between tumor and stromal cells remains poorly understood. Here we present a simple spheroid invasion assay to assess the role of CAFs in the collective migration of epithelial tumor cells. This method enables the characterization of 3D spheroid invasion patterns through live cell fluorescent labeling combined with spinning disc microscopy. When embedded in extracellular matrix, the invasive strands of spheroids can be tracked and leader/follower organization of CAFs and cancer cells can be quantified.

Keywords
3d spheroid invasioncancer associated fibroblastscollective migrationdisseminationepithelial cancer cellsleader/follower cells3d spheroid invasionCancer associated fibroblastsCancer-associated fibroblastsCell culture techniquesCell line, tumorCell movementCell trackingCollective invasionCollective migrationEpithelial cancer cellsExtracellular matrixHumansImaging, three-dimensionalLeader/follower cellsMicroscopy, fluorescenceSpheroids, cellularTumor cells, cultured

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Crispr Knock-Ins In Organoids To Track Tumor Cell Subpopulations, Q4 Agency Scopus (SJR), its regional focus and specialization in Genetics, give it significant recognition in a specific niche of scientific knowledge at an international level.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-24, the following number of citations:

  • WoS: 15
  • Scopus: 15
  • Europe PMC: 12
  • OpenCitations: 13
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-24:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 69.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 69 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 28.35.
  • The number of mentions on the social network X (formerly Twitter): 53 (Altmetric).
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Conti, Sefora) and Last Author (Labernadie, Anna).

the authors responsible for correspondence tasks have been Conti, Sefora and Trepat, Xavier.