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Grant support

The authors were supported by grants from the Deutsche Forschungsgemeinschaft (SFB TRR57, CRC1382). The MICROB-PREDICT, GALAXY and LIVERHOPE projects have received funding from the European Union's Horizon 2020 research and innovation programme under grant agreements numbers 825694, 668031 and 731875 respectively. The manuscript reflects only the authors' views, and the European Commission is not responsible for any use that may be made of the information it contains. The funders had no influence on study design, data collection and analysis, decision to publish or preparation of the manuscript. The conference was supported by grants from the Deutsche Forschungsgemeinschaft (SFB TRR57) (20.000sic), EASL (10.000sic) and EFCLIF (10.000sic). An unrestricted grant of 850sic was obtained by Bayer.

Analysis of institutional authors

Trebicka, JonelCorresponding Author
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Review

Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview

Publicated to:International Journal Of Molecular Sciences. 21 (6): 2027- - 2020-03-02 21(6), DOI: 10.3390/ijms21062027

Authors: Torres, S; Abdullah, Z; Brol, MJ; Hellerbrand, C; Fernandez, M; Fiorotto, R; Klein, S; Königshofer, P; Liedtke, C; Lotersztajn, S; Nevzorova, YA; Schierwagen, R; Reiberger, T; Uschner, FE; Tacke, F; Weiskirchen, R; Trebicka, J

Affiliations

12 Octubre Hlth Res Inst IMAS12, Madrid 28040, Spain - Author
Charite Univ Med Berlin, Dept Gastroenterol & Hepatol, D-10117 Berlin, Germany - Author
Ctr Rech Inflammat, Inserm UMR 1149, F-75890 Paris, France - Author
European Fdn Study Chron Liver Failure, Barcelona 08021, Spain - Author
Externs. Institute for Bioengineering of Catalonia - Author
Friedrich Alexander Univ Erlangen Nurnberg, Inst Biochem, D-91054 Erlangen, Germany - Author
Goethe Univ Frankfurt, Dept Internal Med 1, D-60590 Frankfurt, Germany - Author
Hop Beaujon, AP HP, Dept Hosp Univ UNITY, Serv Hepatol, F-92110 Clichy, France - Author
Inst Bioengn Catalunya, Barcelona 08028, Spain - Author
Institute for Bioengineering of Catalonia - Author
Med Univ Vienna, Dept Internal Med 3, Div Gastroenterol & Hepatol, A-1090 Vienna, Austria - Author
Med Univ Vienna, HEPEX Lab Liver Fibrosis & Portal Hypertens, A-1090 Vienna, Austria - Author
Rhein Westfal TH Aachen, Univ Hosp, Dept Internal Med 3, D-52074 Aachen, Germany - Author
Univ Barcelona, IDIBAPS Biomed Res Inst, Angiogenesis Liver Dis Res Grp, Barcelona 08036, Spain - Author
Univ Clin, Dept Internal Med 1, D-53127 Bonn, Germany - Author
Univ Clin, Inst Mol Med & Expt Immunol, D-53127 Bonn, Germany - Author
Univ Complutense Madrid, Dept Genet Physiol & Microbiol, Fac Biol, Madrid 28040, Spain - Author
Univ Hosp Aachen, IFMPEGKC, Inst Mol Pathobiochem Expt Gene Therapy & Clin Ch, D-52074 Aachen, Germany - Author
Univ Paris Diderot, Fac Med, Lab Excellence Inflamex, Sorbonne Paris Cite, Site Xavier Bichat, F-75018 Paris, France - Author
Univ Southern Denmark, DK-5230 Odense, Denmark - Author
Yale Univ, Sch Med, Yale Liver Ctr, Dept Internal Med, New Haven, CT 06520 USA - Author
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Abstract

Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.

Keywords
fibrogenesishepatic stellate cellshepatocellular cancerin vitro modelsBileBiliary epithelial-cellsCholine-deficient dietEndothelial growth-factorFibrogenesisFibrotic liverHepatic stellate cellsHepatocellular cancerHepatocytesIn vitro modelsMiceRat-liverRodent cholangiocytesSteatosis

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal International Journal Of Molecular Sciences due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2020, it was in position 67/295, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.6, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-24, the following number of citations:

  • WoS: 10
  • Scopus: 12
  • Europe PMC: 6
  • OpenCitations: 11
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-24:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 59.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 59 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.75.
  • The number of mentions on the social network X (formerly Twitter): 5 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Austria; Denmark; France; Germany; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Trebicka, Jonel).

the author responsible for correspondence tasks has been Trebicka, Jonel.