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Analysis of institutional authors

Pegueroles MAuthorAguirre AAuthorEngel EAuthorPlanell JAuthorAparicio CCorresponding Author
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Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: A gene expression study using real-time RT-PCR

Publicated to:Journal Of Materials Science-Materials In Medicine. 22 (3): 617-627 - 2011-03-01 22(3), DOI: 10.1007/s10856-011-4229-3

Authors: Pegueroles, M; Aguirre, A; Engel, E; Pavon, G; Gil, FJ; Planell, JA; Migonney, V; Aparicio, C

Affiliations

Biomaterials for Regenerative Therapies. Institute for Bioengineering of Catalonia - Author
Inst Bioengn Catalonia IBEC, Barcelona 08028, Spain - Author
Inst Galilee, Lab Biomat & Polymeres Special, UMR 7052, F-93430 Villetaneuse, France - Author
Institut Galilée - Author
Institute for Bioengineering of Catalonia - Author
Instituto de Bioingeniería de Cataluña - Author
Univ Politecn Cataluna, Biomat Biomech & Tissue Engn Grp, Dept Mat Sci & Met, E-08028 Barcelona, Spain - Author
Universitat Politècnica de Catalunya - Author
University of Minnesota Twin Cities - Author
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Abstract

Biomaterial surface properties, via alterations in the adsorbed protein layer, and the presence of specific functional groups can influence integrin binding specificity, thereby modulating cell adhesion and differentiation processes. The adsorption of fibronectin, a protein directly involved in osteoblast adhesion to the extracellular matrix, has been related to different physical and chemical properties of biomaterial surfaces. This study used blasting particles of different sizes and chemical compositions to evaluate the response of MG63 osteoblast-like cells on smooth and blasted titanium surfaces, with and without fibronectin coatings, by means of real-time reverse transcription-polymerase chain reaction (qRT-PCR) assays. This response included (a) expression of the α5, αv and α3integrin subunits, which can bind to fibronectin through the RGD binding site, and (b) expression of alkaline phosphatase (ALP) and osteocalcin (OC) as cell-differentiation markers. ALP activity and synthesis of OC were also tested. Cells on SiC-blasted Ti surfaces expressed higher amounts of the α5 mRNA gene than cells on Al2O 3-blasted Ti surfaces. This may be related to the fact that SiC-blasted surfaces adsorbed higher amounts of fibronectin due to their higher surface free energy and therefore provided a higher number of specific cell-binding sites. Fn-coated Ti surfaces decreased α5 mRNA gene expression, by favoring the formation of other integrins involved in adhesion over α5β1. The changes in a5 mRNA expression induced by the presence of fibronectin coatings may moreover influence the osteoblast differentiation pathway, as fibronectin coatings on Ti surfaces also decreased both ALP mRNA expression and ALP activity after 14 and 21 days of cell culture. © Springer Science+Business Media, LLC 2011.

Keywords
Alkaline phosphataseBinding sitesBiocompatible materialsCell adhesionCell differentiationCell lineFibronectinsHumansIntegrin alpha3beta1Integrin alphavbeta3OsteoblastsOsteocalcinPhenotypeReverse transcriptase polymerase chain reactionTitanium

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Materials Science-Materials In Medicine due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2011, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Biomedical Engineering.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.05, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions May 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-05-22, the following number of citations:

  • WoS: 24
  • Scopus: 27
  • Europe PMC: 12
  • OpenCitations: 26
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-22:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 39 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Pegueroles, Marta) and Last Author (Aparicio, Conrado).

the author responsible for correspondence tasks has been Aparicio, Conrado.